Purified 抗 STAT3 Phospho (Tyr7

Purified anti-STAT3 Phospho (Tyr705) Antibody详细如下：

Description ：Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM,EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediatingexpression of a variety of genes in response to the stimuli.

Verified Reactivity ：Human, Mouse

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：KLH conjugated modified synthetic peptide

Formulation ：This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.

Preparation ：The antibody was purified by affinity chromatography.

Concentration ：0.5 mg/ml

Storage & Handling ：Upon receipt, store undiluted between 2°C and 8°C.

Application ：WB - Quality tested、ICC, ChIP - Verified

Recommended Usage ：Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 0.5 - 1.0 µg per ml (1:500 - 1:1000 dilution). Forimmunocytochemistry, a concentration of 0.5 μg/ml is recommended. For ChIP applications, thesuggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimalperformance for each application.

Application Notes ：The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation ofhuman STAT3 protein and has been shown to be useful for Western blotting.

During PD testing, this clone failed to stain PFA-fixed cells that had been permeabilized with TritonX-100. We highly recommend permeabilizing cells with methanol.