TotalSeq™-C0020 抗人 CD270 (HVEM

TotalSeq™-C0020 anti-human CD270 (HVEM, TR2) Antibody详细如下：

Description ：The 122 antibody recognizes human HVEM also known as herpesvirus entry mediator A, tumornecrosis factor receptor superfamily, member 14, TNFRSF14, and tumor necrosis factorreceptor like 2. HVEM, a member of the TNFR superfamily, is a type I transmembrane proteincontaining 2 TNF receptor domains with a predicted molecular weight of approximately 30 kD.HVEM is widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen,thymus and other organs. Resting T cells and naïve and memory B cells express high levels ofHVEM as well. In humans, HVEM is not expressed in germinal center B cells. Immature dendriticcells express high levels of HVEM that is downregulated upon maturation. HVEM plays animportant role in herpes simplex virus pathogenesis by enhancing entry into cells. Signalingthrough HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response toinfection or cellular stress and activate the immune response. HVEM binds to LIGHT and hasalso been shown to associate with several other proteins including TRAF1, TRAF2, TRAF3,TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha.

Verified Reactivity ：Human

Reported Reactivity ：African Green, Baboon, Cynomolgus, Rhesus

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Recombinant human HVEM protein

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.

Concentration ：0.5 mg/ml

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application ：PG - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.