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- 齐源
- 151202
- 国产/进口
- 2025年07月10日
- IHC-F, ICC
- 大鼠
- 人、小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Ki-67
- 级别：
mouse/human Ki-67 Antibody
- 目录编号：
mouse/human Ki-67 Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
mouse/human Ki-67 Antibody
- 抗体英文名：
Purified anti-mouse/human Ki-67 Antibody
- 抗体名：
mouse/human Ki-67 Antibody
- 标记物：
mouse/human Ki-67 Antibody
- 宿主：
大鼠
- 适应物种：
人、小鼠
- 免疫原：
mouse/human Ki-67 Antibody
- 亚型：
mouse/human Ki-67 Antibody
- 形态：
mouse/human Ki-67 Antibody
- 应用范围：
IHC-F, ICC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
100 µg
Description ：The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which wasgenerated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 proteinplays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable.Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic markerin various tumors.
Verified Reactivity ：Mouse, Human
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinantprotein.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C.
Application ：IHC-F - Quality tested、ICC - Verified
Recommended Usage ：Each lot of this antibody is quality control tested by immunocytochemistry. Forimmunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. Forimmunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 -5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验1. Su T, et al. 2021. Metabolites. 11:. PubMed
2. Miyauchi K, et al. 2021. Front Immunol. 12:663177. PubMed
3. Flores–Santibáñez F, et al. 2018. Front Immunol. 0.520138889. PubMed
4. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
5. Schrage R, et al. 2015. Nat Commun. 6: 10156. PubMed
6. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
7. Reyes RV, et al. 2022. Front Neurosci. 16:908719. PubMed
8. Coover RA, et al. 2018. Acta Neuropathol Commun. 6:127. PubMed
9. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
10. Lee YS, et al. 2018. Cell Host Microbe. 24:833. PubMed
11. Ren J, et al. 2018. Cell Cycle. 1.25. PubMed
【讨论】抗体和那些不能说的"秘密"-抗体选择的PASS标准 (请加精)
专家观点 哥本哈根大学Dr.John Couchman认为优质抗体，尤其是多抗，应该是2步纯化： Not only must I affinity purify the antibody but also perform antigen adsorption on my tissues sections. Unfortunately, many commercial antibodies are not affinity purified from serum
the sample briefly and load onto your SDS-PAGE gel.Add Dithiothreitol （DTT） or β-mercaptoethanol （2-ME） to the Trident Sample Buffer before use to reduce proteins, if necessary.电泳和转膜1. Load 30 μg of each protein extract or 100 ng of purified protein
请教前辈，我要做westernblot及免疫组化，抗原是人源性的PEDF，实验动物是大鼠，以下有两种santa cruz公司的抗体，source及specificity的说明不同，请问我选用哪一种更为适合，两者有何区别阿？谢谢指教！！！！ SOURCE PEDF (H-125) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 281-405
