- ￥3900 - 5200
- 齐源
- 504125
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- 2025年07月14日
- ICFC
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- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
IL-4
- 级别：
mouse IL-4 Antibody
- 目录编号：
mouse IL-4 Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
mouse IL-4 Antibody
- 抗体英文名：
Brilliant Violet 605™ anti-mouse IL-4 Antibody
- 抗体名：
IL-4 抗体
- 标记物：
mouse IL-4 Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 亚型：
mouse IL-4 Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃1
- 浓度：
0.2 mg/mL
- 规格：
125 µL/50 µg
| 规格： | 125 µL | 产品价格： | ￥3900.0 |
|---|---|---|---|
| 规格： | 50 µg | 产品价格： | ￥5200.0 |
Description ：IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is apotent lymphoid cell growth factor which stimulates the growth and activation of certain B cellsand T cells. IL-4 is important for regulation of T helper subset development.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Partially purified native mouse IL-4
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™under optimal conditions.
Concentration： µg sizes: 0.2 mg/mL µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lotnumber in our Certificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.4 µgper million cells in 100 µl volume. For flow cytometric staining, the suggested use of this reagent is5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommendedthat the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setupare appropriate for detecting this channel. Refer to your instrument manual or manufacturerfor support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISACapture)
6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:SpringerVerlag. (IHC)
8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
16. Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
Product Citations：
1. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
2. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
3. Lorentsen KJ, et al. 2018. Nat Commun. 9:1679. PubMed
4. Li A, et al. 2020. J Virol. 94:00:00. PubMed
5. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
6. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
实验原理 亚适剂量抗IgM抗体对B淋巴细胞的刺激较弱，当加人IL-4时，B淋巴细胞对亚适剂量抗IgM抗体刺激表现出明显的DNA合成增加，3 H-TdR掺人量与IL-4含量相关。 实验步骤 1. 小鼠脾脏B细胞悬液的制备 1) 取BALB／c或C57BL／6小鼠，常规方法分离脾脏淋巴细胞。用Hanks液洗2次；再用10％NBS-IMDM培养液调整细胞浓度为1X107 ～2X107 ／
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠白介素-4 (IL- 4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 IL-4 单抗包被于酶标板上，标准品和样品中的 IL-4与单抗结合，加入生物素化的抗小鼠
