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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD70
- 级别：
CD70 Antibody
- 目录编号：
CD70 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD70 Antibody
- 抗体英文名：
TotalSeq™-C0027 anti-human CD70 Antibody
- 抗体名：
CD70 抗体
- 标记物：
CD70 Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD70 Antibody
- 亚型：
CD70 Antibody
- 形态：
CD70 Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
10 µg
Description ：CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member ofthe tumor necrosis factor superfamily. CD70 is expressed on activated T, B and NK cells,activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia andlarge B cell lymphomas. CD70 costimulates T cell proliferation and differentiation. It plays a rolein the pDC-induced B cell differentiation. The ligand of CD70 is CD27.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：CD70-transfected L cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Shaw J, et al. 2010. Blood 115:3051. (Block)
Product Citations：
1. Bachireddy P, et al. 2021. Cell Rep. 37:109992. PubMed
CD70 分子 CD70 常用单克隆抗体或代号： Ki- 24;(CD27L) 主要表达细胞： Tsub,Ba,RS [NL] 分子质量(kDa)和结构： gp55,75,95,110,170 功 能： CD27的配体,淋巴细胞活化 CD70 Aka CD27 ligand Role in T cell activation; induces T cell
（一）材料： 1．动物：家兔 2．人血清 3．羊毛脂、石蜡油、卡介苗 4．其它：无菌生理盐水，无菌研钵等 （二）方法： 1．弗氏 佐剂 的制备 将羊毛脂与石蜡油按1：5比例混合，高压灭菌。 2．人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中，边研磨边滴加等量的含卡介苗3-4mg/mL的抗原
抗人肺腺癌单链抗体的构建 (1)将表达载体pFUW80质粒经XbaI和EcoRI双酶切，同样双酶切在pUC19中克隆的VL基因，分别回收后，连接并转化大肠杆菌JM109株，鉴定阳性重组子(可通过XbaI、EcoRI双酶切切下合适大小的带，即VL片段)。 (2)将上述经过鉴定的重组质粒用XhoI、SpeI双酶切，回收后，与同样双酶切的在pUC19中克隆的VH基因相连，再次转化JM109感受态细菌。重组质粒筛选、鉴定同上所述。至此抗人
