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96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin (FE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin (FE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
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文献和实验相关实验
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
系统照相。 超声波裂解细菌 渗透休克细菌沉淀加1/10培养体积20 mM Tris-HCl（pH 7.5，0℃）重悬，冰浴，超声波裂解，20%工作选择，脉冲粉碎1 min，然后13000 r/min离心5 min，-20℃保存上清、细菌沉淀。上清进行TCA沉淀，处理同上。沉淀用Protein Extraction Reagent（Ni-NTAHi Bind Purification Kit BugBuster. 提供 ）按 Novagen公司《pET System Manual》提供方法反复处理
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Chicken Ferritin (FE) ELISA Kit
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