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文献和实验Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
of Small‐Molecule Inhibitors of In Vivo Protein‐Protein Interactions by Genetic Selection Support Protocol 1: Construction and Optimization of a Strain for Inhibitor Selection Support Protocol 2: Construction
【资源】[共享]Adhesion Protein Protocols——（很有用的）
Materials 2.1— Construction of Adhesion Molecule—Ig Chimeras 2.2— Electroporation of Cells 2.3— Harvest and Culture of Transfectant Clones 2.4— Selection (ELISA) and Subcloning 2.5— Purification of Chimeric Proteins 3— Methods 3.1—
on the cell surface, facilitating cell sorting, and because they are involved in important aspects of tumor biology such as cell proliferation, adhesion, or migration. After three rounds of selection with the 3ZF library and four rounds with the 6ZF
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