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文献和实验Activation of MAPKs by G Protein-Coupled Receptors
, thereby activating or inhibiting the production of a variety of second messengers, such as cyclic adenosine monophosphate, cyclic guanosine monophosphate, diacylglycerol, inositol triphosphate (IP3 ), and phosphatidyinositol triphosphate (PIP3 ), arachidonic
Measurement of Phospholipid Metabolism in Intact Neutrophils
-kinase (PI3-K) in intact neutrophils. PLA2 activity is measured as the release of radiolabed arachidonic acid. PLC activity is measured as the accumulation of inositol 1,4,5-trisphosphate (IP3 ), a water-soluble product, using a commercially available
些含氮激素的作用信息并不以cAMP为媒介进行传递,如胰岛素、催产素、催乳素、某些下丘脑调节肽和生长因子等。实验证明,这些激素作用于膜受体后,往往引起细胞膜磷脂酰肌醇转变成为三磷酸肌醇(inositol-1,4,5,triphosphate,IP3 )和二酰甘油(diacylglycerol,DG),并导致胞浆中Ca2+ 浓度增高。近年来,有人提出IP3 和DG可能是第二信使的学说引起人们的重视,并且得到越来越我的实验证实。这一学说认为,在激素的作用下,可能通过G蛋白的介导,激活细胞膜内的磷脂
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