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文献和实验Methods to Detect Protein Glutathionylation
role in mediating redox?sensitive signaling. Indeed, glutathionylation can affect protein function by altering activity, protein?protein interactions, and ligand binding. Glutathionylation may also serve to prevent cysteine residues from undergoing
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
reporter plasmids (TCF reporter plasmid kit; Upstate) is needed. B Pharmacological inhibition ofGSK-3 activity. MiaPaCa2 pancreatic cancer cells do not express E-cadherin and there is free (free from binding to Ecadherin)cytosolic β-catenin pool
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