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文献和实验). In this case, the "bait" protein, Borg3, was produced as a glutathione S -transferase (GST) fusion in E. coli and then mixed with NIH 3T3 cell lysate. Four interacting proteins were identified by mixed-peptide sequencing: heat shock protein Hsp70
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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