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文献和实验Genetic-Engineered Models of Skeletal Diseases I: Collagen Type X
transgenesis, and from murine and human genetics.
1 to a tube. 2) Add 20 μl of Antibody Mix. 3) Mix well and incubate for 20 min at 2–8°C. 4) Wash the cells by adding 2 ml Buffer 1. Mix well by tilting the tube several times and centrifuge at 300 x g for 10 min at 2–
-HCL PH 6.6, and that is better) Buffer PE 10 mM Tris-HCl pH 7.5 80% ethanol Buffer QBT equilibration buffer 750 mM NaCl 50 mM MOPS pH 7.0 15% isopropanol 0.15% triton X-100 Buffer QC wash buffer
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