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文献和实验【原创】自己总结的RNAi干预的癌基因(75条) 大家一起来补全好吗
melanoma BRAF(V599E) (206) Brk (207) CDK-2 (138) CEACAM6 #2 #4 COX-2 #8 c-raf #8 c-Kit Signal transduction Gastrointestinal CSF-1, CSF-1 receptor (c-fms) CXCR4 (163) Cyclin E Cyclin E Cell-cycle control Hepatocarcinoma EGFR EGFR (209, 210
PriCells: Introduction of Isolation and Culture of Human Primary Hepatocytes
. This latter isolation yielded very poor quality hepatocytes. The chemical and mechanical dissociation methods are discussed at length in a review. The development of a technique utilizing enzymes to digest the liver providing a high yield of functionally active rat
v468 Chapter 9 自身启动子报告基因验证转录靶点
,Ontario, Canada). 5. 2.5 U/μL Pfu DNA polymerase (e.g. from Fermentas), suppliedwith appropriate 10× reaction buffer. 6. 96% Tetramethylene sulfoxide (TMSO) (e.g. from Sigma,St. Louis, MO). 7. Qiaquick PCR Purification Kit (Qiagen) or similar kit
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