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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
, and twice for 10 min each at 65°C in a solution of 0.1 x SSPE and 0.1% SDS. It was wrapped with Saran Wrap and exposed to an imaging plate (Fuji Film, Tokyo). The plate was read in an imaging analyzer (BAS 2000II, Fuji Film). In place of the imaging plate
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
reactive oxygen intermediates in rat mast cells 113 and to measure hydrogen peroxide in endothelial cells.112 Dihydrorhodamine 6G (D633, Figure 12.27) is another reduced rhodamine that has been shown to be taken up and oxidized by live cells.114�116
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
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