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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1 ), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human
Mast Cell Histamine and Cytokine Assays
RI) on cord bloodderived mast cells by myeloma IgE and anti-human IgE is used to induce histamine release. Histamine levels were measured in the culture supernatants using an enzymelinked immunosorbent assay. A human mast cell line (HMC-1), derived
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