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文献和实验Translocation Assays of Protein Kinase C Activation
response, such as neurodegeneration, is to identify the endogenous PKC isoforms that become “membrane-associated” in response to an appropriate stimulus. Translocation assays have classically been used to screen the individual
Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry
a dual fluorescent HCMV, where the capsid-associated tegument protein pp150 is labelled with EGFP and the envelope glycoprotein gM with mCherry. This dual labelled virus allows for the separate tracking of the viral envelope fusing with a cellular
Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
with altered response to growth factor signals. The ubiquitous mitogen-activated protein kinase (MAPk) cascade, which transmits growth factor signals from the cell membrane to the nucleus, provides a principal mechanism for regulation of cell cycle progression
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