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文献和实验In Vitro DNA Mismatch Repair in Human Cells
The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor
Strategies for Cloning Mammalian DNA Repair Genes
During the last 20 years, the cloning and identification of DNA repair genes in bacteria, yeast, human, mouse, and other organisms have been an expanding enterprise. Gene/cDNA isolation and analysis is a critical step toward discovering gene
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
DNA (Stratagene) Plasmid Midi kit (QIAGEN) Proteinase K (Roche Applied Science) Recombinant Hop2-Mnd1 complex, purified (5 µM) or recombinant human Rad54, purified (5 µM) (see Step 5) Purify Hop2-Mnd1 complex
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