- ￥2100 - 7100
- 齐源
- 405753
- 国产/进口
- 2025年07月07日
- FC
- 大鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
9999
- 靶点：
IgD
- 级别：
mouse IgD Antibody
- 目录编号：
mouse IgD Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
mouse IgD Antibody
- 抗体英文名：
Spark Violet™ 423 anti-mouse IgD Antibody
- 抗体名：
IgD 抗体
- 标记物：
mouse IgD Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
mouse IgD Antibody
- 亚型：
mouse IgD Antibody
- 形态：
mouse IgD Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/mL
- 规格：
25 ug/100 ug
| 规格： | 25 ug | 产品价格： | ￥2100.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥7100.0 |
Spark Violet™ 423 anti-mouse IgD Antibody详细如下：
Description ：Surface IgD is an important B cell differentiation marker.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423under optimal conditions.
Concentration ：0.5 mg/mL
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per millioncells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance foreach application.
* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.
Excitation Laser ：Violet Laser (405 nm)
Description ：Surface IgD is an important B cell differentiation marker.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423under optimal conditions.
Concentration ：0.5 mg/mL
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per millioncells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance foreach application.
* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation)：
1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
相关实验
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
文献支持
Spark Violet™ 423 抗小鼠 IgD 流式抗体
￥2100 - 7100
