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- 2025年07月14日
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Transgenic Mouse and Transgenic Rat Outline
with any questions or Meet the Staff. 2. Plan the experiment. What is the purpose of your experiment? Do you want to define tissue specific regulatory sequences? Do you want to overexpress a protein in a specific cell lineage? You will need to obtain or clone
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
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