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- 2025年07月11日
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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
with patterns that match those of rodent T1α. However, the human data are unclear, because antibodies to rodent T1α do not recognize ATI cell antigens in normal adult human lung. This likely indicates that O-glycosylation of the human protein(s
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
as lung or tumour induced, we determined their currently known membrane-associated structure (bilayer spanning versus lipid anchor (intra- or extracellular) versus peripheral interaction) from scientific reports and/or protein databases
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