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- Biomatik
- 美国
- EKU06137
- 2025年07月12日
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
molecule with 3′ T overhangs on either side of the insert site. This allows cohesive-end ligation of the PCR product with the pCRII vector. In this chapter, I describe the steps that I followed in order to clone a 576-bp fragment of the rat N -cadherin gene
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