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文献和实验Isolation and Myogenic Differentiation of Mesenchymal Stem Cells for Urologic Tissue Engineering
or specific myogenic growth factors (PDGF-BB, HGF, TGF-β). These differential cells can migrate to a scaffold for differentiation into smooth muscle-like cells in vivo. Furthermore, stem cell-seeded scaffolds that are implanted into the bladders repopulate
Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.
Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells
between investigators, many methods are employed to measure the extent of differentiation. These methods include, but are not limited to, reverse transcriptase PCR (RT-PCR) for detection of osteogenic transcripts, enzyme linked immunosorbent assay (ELISA) for secreted
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