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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
medium for OP9 cells (for Protocols 1, 2) Leukemia inhibitory factor (LIF; 10 µg/mL; Chemicon LIF2010) (for Protocol 2; see Step 20) Lympholyte-M (Cedarlane CL5120) (for Protocol 3) Mice, 4-8 wk old (for Protocol 4; see Step 69) Mice, fetal
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
our current knowledge regarding the isolation human bone marrow?derived progenitor cells for the paracrine stimulation of islet regeneration after transplantation into immunodeficient mouse models of diabetes. By using high aldehyde dehydrogenase (ALDHhi
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