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文献和实验Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors
Leucine-rich repeat-containing G protein-coupled receptors (LGRs) are type A rhodopsin-like GPCRs that include the glycoprotein pituitary hormone receptors for the thyroid-stimulating, follicle-stimulating, and luteinizing hormones (TSH, FSH
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
. 21. Incubate for 30 min at room temperature. Enrichment of Ligation Fragments Containing the Protein of Interest in the Complex 22. Remove 10 µL from the sample (from Step 21). Store at -20°C. This sample will serve
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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