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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
can destroy the protein. Use 25 µl of 0.41 mg/ml NGFNgf Standards ITube #NGF0.05% Acetic AcidFinal Conc.125 µl975 µl10 µg/ml2100 µl #19.9 ml100 ng/ml2.5100 µl #2900 µl10 ng/ml3100 µl #29.9 ml1 ng/mlUse the above solutions to make the following more dilute
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