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文献和实验Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
Establishment of Stable Transfectant of CHO Lec Cells
mannose type, but differ in the number of mannoses, ranging from Man9 to Man5 . O-glycosylation is homogenous, with only a single GalNAc residue attached per site. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl
Establishment of Stable Transfectant of CHO Lec Cells
. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose
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