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文献和实验A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
that bind to human fatty acid amide hydrolase (FAAH). This SPA assay utilizes the specific binding of [3 H]-R (+)-methanandamide (3 H-MAEA), a competitive nonhydrolyzed FAAH inhibitor, to FAAH expressing microsomes and evaluates its displacement by FAAH
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
. Moerke, N.J. 2009. Fluorescence polarization (FP) assays for monitoring peptide‐protein or nucleic acid‐protein binding. Curr. Protoc. Chem. Biol. 1:1‐15.
An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA
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