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文献和实验Metabolic Labeling with Fatty Acids
of radiolabeled fatty acids (e.g., [3 H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein?bound
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
for mapping distributions of chromosome-associated proteins, identifying and characterizing cis -regulatory DNA elements bound by particular proteins, and mapping functional protein domains necessary for chromosomal binding or other activities
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
an alternate approach, the Nucleic Acid Programmable Protein Array (NAPPA) is constructed by spotting protein?encoding plasmid DNA at high density, in addressable fashion, on an array surface. Proteins are subsequently generated in situ
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