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文献和实验Use of Animal Models to Understand Human Muscular Dystrophy
In the decade following the identification of mutations in the dystrophin genein Duchenne (1 ) and Becker (2 ) muscular dystrophies (DMD/BMD), defects in components of the dystrophin-glycoprotein complex (DGC), which links F-
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
markers, specific for type I cells, have been described in the recent past. The first one was T1α, a 36-kDa glycoprotein found in rodent lungs. Antibodies have been developed against lung proteins that specifically label type I cells in human lung
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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