Human DNA Methyltransferase 1

DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo

for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation

表观遗传修饰：想要改变基因表达？不只有 DNA，染色体也可以！

动物基因组中，CpG 岛位于基因启动子上，使基因就处于“待命”状态。CpG 岛是调控基因表达的“开关”[1][7]。     图 3. DNA 甲基化模式[8]。     DNA 去甲基化 (DNA demethylation) 可分为被动去甲基化与主动去甲基化。 被动去甲基化是由于 DNMT1 功能被抑制而引起的，发生在 DNA 复制过程。 主动去甲基化：通过经典通路 TET-TDG-BER 将 5mC 氧化并移除可实现主动去甲基化，不依赖于 DNA 复制。    

Detection of Methyl′Sensitive DNA′Binding Proteins with Possible Involvement in the Imprinting Phenomenon

methyltransferase 1 (Dnmt1) 1 . Recently two new methyltransferases have been identified, Dnmt2 and Dnmt3 2 ,3 , but it remains unclear which of these enzymes has de novo activity and differentially methylates imprinted genes. Parent′specific methylation patterns