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- 2025年07月14日
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文献和实验ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
interactions involve the simultaneous binding of two ligands to the same receptor. An allosteric modulator causes a conformational change in the receptor protein that yields a change in the binding or signaling of an orthosteric agent, i.e., an agonist
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