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文献和实验Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Assessment of CYP3A4 Time-Dependent Inhibition in Plated and Suspended Human Hepatocytes
This chapter provides a step-by-step description of methodology used to assess time-dependent inhibition/inactivation (TDI) potential of cytochrome P450 3A4 (CYP3A4) by a test compound using human hepatocytes in a 96-well plate format. Human
Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6-Hydroxylation
% of total human cytochrome P450 content in adult liver (3 ), although large inter-individual differences exist in hepatic CYP3A content. CYP3A4 is present in all adult human livers and is inducible by drugs such as rifampin (rifampicin) and dexamethasone
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