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文献和实验Two-hybrid analysis of genetic
,is expressed from a plasmid containing the HIS3 marker,and the activation domain fused protein,or prey,is expressed from a plasmid containing the TRP1 marker.In the most commonly used bait plasmid,pEG202,the bait is expressed from the constitutive yeast ADH
Two-hybrid analysis of genetic regulatory networks
but absent in the non-interacting Cdks; inspection of an alignment of the Cdk protein sequences suggested residues that may be important for specific interactions with certain Cdis. Second, Cdi3, Drosophila Cyclin D, interacted much more strongly with human
kinase-1 enzyme. J. Biol. Chem. 277(8):6667-6675. Li L, Mao J, Sun L, Liu W, and Wu D. (2002). Second cysteine-rich domain of Dickkopf-2 activates canonical Wnt signaling pathway via LRP-6 independently of Dishevelled. J. Biol. Chem. 277(8):5977-5981
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