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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
PDGFR-α,PDGFR-βSCFR Five immunoglobulin-like family (Kit),CSF-R(Fms),Flk-2 domains extracellularly EGF receptor family EGFR(ErbB),ErbB2(Neu),ErbB3,ErbB4 Two cysteine-rich
, C) DNA-binding ELISA of the selected -6ZF (B) and -3ZF protein domains (C) expressed as fusions with MBP. All TFZF s were selected for VE-cadherin upregulation except 54.3, which was selected for ICAM-1 activation. The DNA substrates contained the 18
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