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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
in the circulation, and their levels are believed to reflect type II collagen synthesis. In this chapter we describe the development of a specific enzyme-linked immunosorbent assay (ELISA) for the measurement of the IIA form of PIINP (PIIANP) in serum based
Native Chromatin Preparation and Illumina/Solexa Library Construction
to chromatin as histones, the chromatin should be cross-linked using formaldehyde and sonicated to 200- to 300-bp length. 22. Resuspend 20-22 x 106 purified human CD4+ T cells from Step 21 in 1 mL MNase digestion buffer kept at room
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