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文献和实验Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
(see recipe ) 6 µM unlabeled rat/human corticotropin‐releasing factor (r/hCRF; Peninsula Labs) in assay buffer Radioligand
G Protein-Coupled Receptors Disrupted in Human Genetic Disease
Genetic variation in G protein-coupled receptors (GPCRs) results in the disruption of GPCR function in a wide variety of human genetic diseases. In vitro strategies have been used to elucidate the molecular pathologies that underlie naturally
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
strain SNS‐126 containing the two‐hybrid reporter and protein domains of interest (or control protein) fused to the bacteriophage repressor DNA‐binding domains ( protocol 2 ) 50 to 200 ng
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