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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)
75?) 1.5mL 10x reconstitution buffer 1.5mL 10x DMEM 12mL rat tail type 1 collagen (>3.8mg/mL) 10N NaOH Glacial acetic acid (in case) Method Pre-chill pipettes
they may be split 1:4 into fresh media. (Fungizone can be omitted from this point onwards). 2. Stage I - Preparation of collagen I from rat tails Note: Protocol for approximately 12-14 adolescent (fresh or frozen) rat tails 1) Prepare rat
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