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文献和实验V294.PART II，Chapter 5 细胞粘附实验（英文版）
variations to thebasic protocols and areas where special attentions are required for successfulapplication of the methods. 2. Materials 2.1. Static Adhesion Assays 1. 96-Well enzyme-linked immunosorbent assay (ELISA) plates (Corning
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
to the SAMPLE BUFFER to increase the amount of NGF removed from the sample. Typically, a 10x dilution (w/v) of tissue samples will give a signal sufficient for detection. Once diluted, samples are homogenized on ice for 30 seconds using a cell sonicator at micro
Craig, C.G., Tropepe, V., Morshead, C.M., Reynolds, B.A., Weiss, S., and van der Kooy, D. 1996. In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J
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