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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
a 200 μL pipette, transfer the cut pieces to a freshly prepared 24-well MEF culture plate containing human iPSC medium. 6) Incubate the MEF culture plate containing the picked colonies in a 37°C incubator with a humidified atmosphere of 5% CO2
Native Chromatin Preparation and Illumina/Solexa Library Construction
) Incubate at room temperature for 45 min. 40. Use MinElute PCR Purification Kit or phenol:chloroform extraction to purify DNA. Elute or resuspend DNA in 30 µL of 1X TE, pH 7.4. 41. Add "A" to 3'' ends
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
. Filipczyk, A.A., Laslett, A.L., Mummery, C., and Pera, M.F. 2007. Differentiation is coupled to changes in the cell cycle regulatory apparatus of human embryonic stem cells. Stem Cell Res. 1:45‐60
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