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- 2025年07月11日
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
should have grown to an appropriate size for transfer. The day before transferring the colonies, prepare MEF culture plates using Attachment Factor-coated 12- or 24-well plates. Note: We typically harvest colonies closer to three weeks to avoid differentiation
Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
Mesenchymal stem cells (MSCs) have been isolated from many tissues including differentiating human embryonic stem cells (hESCs). Derivation of MSCs from hESCs consists of two major steps: differentiation and isolation. In our hands
Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells
to isolate and culture human IFE stem cells (IFESCs) offers fascinating therapeutic potential for skin diseases as well as epithelial tissue engineering. Here we describe a straightforward strategy for generation of β1 integrin+ /CD24− IFESCs from scalp
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