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文献和实验The amiloride-sensitive epithelial Na+ channel (ENaC) is typically composed of three structurally related subunits termed α, β, and γ. We describe methods to determine the functional subunit stoichiometry of ENaC based on a biophysical
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
and of the verotoxin B subunit in Vero cells 30 Our Vybrant Apoptosis Assay Kit #11 (V35116, Section 15.5) utilizes MitoTracker CMXRos in combination with Alexa Fluor 488 annexin V in a two-color assay of apoptotic cells (Figure 15.95). MitoTracker Orange CMTMRos
Quantitation Kit (#F-2962, Molecular Probes, Eugene, OR) TE 12 channel multi-pipettors Computer equipped with Microsoft Excel software Ds-DNA Standards 50 µg/ml 100 µg/ml 250 µg/ml 500 µg/ml µl TE 90 80 50 0 µl ds-DNA (0.5 mg/ml) 10 20 50 100
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