Description :Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85%identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells,bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes andexpress cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity tomigrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play animportant role in the ligand-mediated recruitment of T cells into the skin in mice and humans,specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site ofinjury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucialfor the induction and maintenance of self-tolerance and are present in peripheral tissues suchas skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ Tregulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor andits ligand CCL22 (5).
Preparation :The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™under optimal conditions.
Concentration :µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lotnumber in our Certificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric stainingusing the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. Itis recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.