Brilliant Violet 510™ 抗小鼠 CD3

Brilliant Violet 510™ anti-mouse CD3 Antibody详细如下：

Description ：CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex

Verified Reactivity ：Mouse

Antibody Type ：Monoclonal

Host Species ：Rat

Immunogen ：γδTCR-positive T-T hybridoma D1

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™under optimal conditions.

Concentration ：µg sizes: 0.2 mg/mL

µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lotnumber in our Certificate of Analysis online tool.)

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：FC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggesteduse of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setupare appropriate for detecting this channel. Refer to your instrument manual or manufacturerfor support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Excitation Laser ：Violet Laser (405 nm)