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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
A general protocol for staining cell for cytometry analysis
as described below: *The optimal amount of PI may range between 2–10µl/test depending on cell type and experimental system. 2 µl/test is the recommended starting amount. 4.Gently mix the cells and incubate for 15 min at RT in the dark. *For Annexin V
Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric Focusing
) and liquid-chromatography and tandem mass spectrometry (LC-MS/MS). The MicroRotofor isoelectric focusing (IEF) cell fractionates proteins in free solution according to their isoelectric point (pI). We demonstrate the ability of the MicroRotofor
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