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- 齐源
- 109805
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- 2025年07月12日
- FC
- 小鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45.2
- 级别：
anti-mouse CD45.2 Antibody-FITC
- 目录编号：
anti-mouse CD45.2 Antibody-FITC
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-mouse CD45.2 Antibody-FITC
- 抗体英文名：
anti-mouse CD45.2 Antibody-FITC
- 抗体名：
CD45.2 Antibody
- 标记物：
FITC
- 宿主：
小鼠
- 适应物种：
小鼠
- 免疫原：
anti-mouse CD45.2 Antibody-FITC
- 亚型：
anti-mouse CD45.2 Antibody-FITC
- 形态：
anti-mouse CD45.2 Antibody-FITC
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
50 ug/500 ug
| 规格： | 50 ug | 产品价格： | ￥900.0 |
|---|---|---|---|
| 规格： | 500 ug | 产品价格： | ￥2100.0 |
Description ：CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：B10.S mouse thymocytes and splenocytes
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
2. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
3. Miranda K, et al. 2022. iScience. 25:104994. PubMed
4. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
5. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
6. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
7. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
8. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
9. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
10. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
11. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
12. Burman A, et al. 2007. Blood. 110:1064. PubMed
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
