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- 109813
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- 2025年07月13日
- FC
- 小鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45.2
- 级别：
anti-mouse CD45.2 Antibody
- 目录编号：
anti-mouse CD45.2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-mouse CD45.2 Antibody
- 抗体英文名：
anti-mouse CD45.2 Antibody-APC
- 抗体名：
CD45.2 Antibody
- 标记物：
APC
- 宿主：
小鼠
- 适应物种：
小鼠
- 免疫原：
anti-mouse CD45.2 Antibody
- 亚型：
anti-mouse CD45.2 Antibody
- 形态：
anti-mouse CD45.2 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
25 ug/100 ug
| 规格： | 25 ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥2100.0 |
Description ：CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：B10.S mouse thymocytes and splenocytes
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with APC under optimalconditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protectedfrom prolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser ：Red Laser (633 nm)
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文献和实验1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
3. Guo H, Cooper S, Fried.man A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
4. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
5. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
6. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
7. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
8. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
9. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
10. Boyd DF, et al. 2020. Nature. 587:466. PubMed
11. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
12. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗，这种抗体有单抗的吧？ 用于FCM： 1：20-100，这是说用于流式的稀释倍数，是不是稀释倍数越小越好呢？ 同型对照抗体是不是必须要做的？如何选择阴型同型对照抗体呢！ 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助！ freecell 抗体的稀释倍数是需要优化
荧光标记物的定位分析激光扫描共聚焦显微镜可进行重复性极佳的活细胞或固定细胞的荧光定量分析。利用这一功能可对单个细胞或细胞群的溶酶体、线粒体、DNA、RNA和受体分子含量、成分及分布进行定性和定量测定。激光扫描共聚焦显微镜可以自动将荧光图像与相差图像重叠，以显示荧光标记物在形态结构上的精确定位。在同一细胞或组织标本上需同时检查两种或两种以上抗原时要进行双(多)重荧光染色。可采用直接和间接免疫荧光组织化学方法，将这些荧光抗体(如抗A和抗B)以适当比例混合，加在标本上(也可分别加)孵育后，洗去未
