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99
- 英文名：
BioLegend 933104 PE anti-Lck Phospho (Tyr394)
- 供应商：
北京云肽生物科技有限公司
BioLegend 933104 PE anti-Lck Phospho (Tyr394)
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BioLegend 933104 PE anti-Lck Phospho (Tyr394)
产品简介：
产品品牌：BioLegend
产品货号：933104
产品名称：BioLegend 933104 PE anti-Lck Phospho (Tyr394)
产品规格：100 tests
产品说明：
Clone A18002D (See other available formats)
Regulatory Status RUO
Other Names
Lsk, p56lck, LCK Proto-Oncogene, Src Family Tyrosine Kinase, Tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, T cell-specific protein-tyrosine kinase
Isotype Mouse IgG1, κ
描述
Src家族酪氨酸激酶p56Lck（Lck）是一种非受体酪氨酸激酶，在胸腺内T细胞的选择和成熟以及成熟T细胞的功能中起着关键作用。Lck与CD4和CD8表面受体的胞质结构域组成性结合，在T细胞受体（TCR）信号传导中发挥重要作用。TCR与肽抗原负载的MHC复合物的结合导致CD4-和CD8-结合的Lck募集到TCR/CD3信号复合物。然后，Lck将TCRγ链和CD3亚基反磷酸化，从而激活TCR/CD3信号通路，并导致Lck对Zap70的募集和随后的磷酸化。Lck在调节T细胞增殖反应的白细胞介素2信号传导中也起着重要作用。CSK在酪氨酸505对Lck的磷酸化负调控激酶，并被认为产生蛋白质的封闭、无活性构象。相反，Lck激活环中酪氨酸394的磷酸化极大地刺激酶活性。在许多类型的癌症中，Lck是增殖和抗凋亡的驱动因素，并已被提议作为治疗干预的靶点。
The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.
Product Details
Isotype Control PE Mouse IgG1, κ Isotype Ctrl (ICFC)
Verified Reactivity Human, Mouse
Antibody Type Monoclonal
Host Species Mouse
Immunogen
Synthetic peptide corresponding to human Src phosphorylated at tyrosine 419
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application ICFC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Antigen Details
Structure
Lck is a 509 amino acid protein with a predicted molecular weight of 58 kD
Distribution Plasma-membrane associated/T cells
Function Protein Tyrosine Kinase
Cell Type T cells
Biology Area Cell Biology, Signal Transduction
Molecular Family Protein Kinases/Phosphatase
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文献和实验相关实验
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known
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BioLegend 933104 PE anti-Lck Phospho (Tyr394)
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