BioLegend 836804 Purified anti

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BioLegend 836804 Purified anti-Rat Blood-Brain Barrier
产品简介：
产品品牌：BioLegend
产品货号：836804
产品名称：BioLegend 836804 Purified anti-Rat Blood-Brain Barrier
产品规格：100 µg

产品说明：
Clone   SMI 71 (See other available formats)
Regulatory Status   RUO
Other Names   Endothelial barrier antigen, EBA
Isotype   Mouse IgM, κ

描述
SMI 71对在具有血脑屏障或血神经屏障的区域中发现的大鼠内皮蛋白具有特异性。抗体不与心室周围器官的内皮细胞或外周组织中的开窗内皮细胞反应。具体地，已经表明，在肝脏的血管和窦、心脏的血管、肾上腺、骨骼肌、肠、胸腺、淋巴结、胰腺、甲状腺或皮肤中没有观察到SMI 71的染色。然而，在脾脏的一些血管壁和皮肤的表皮上观察到斑片状反应3。新生大鼠对抗体的反应性随着血脑屏障的成熟而发展。实验性变态反应性脑脊髓炎病变的反应性消失。与星形胶质细胞和可能的神经元对建立血脑屏障至关重要的观点相反，神经元（由SMI 311和抗MAP2监测）和星形胶质细胞（由抗GFAP和抗S100B监测）的破坏仅导致SMI 71反应性的短暂消除和血清白蛋白的短暂跨内皮通道。
SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.

Product Details
Verified Reactivity   Rat
Antibody Type    Monoclonal
Host Species   Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography.
Concentration    0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application    IHC-P - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers

以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
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