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99
- 英文名：
Recombinant Human IL-6Rα (carrier-free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 751504 Recombinant Human IL-6Rα (carrier-free)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 751504 Recombinant Human IL-6Rα (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：751504
产品名称：BioLegend 751504 Recombinant Human IL-6Rα (carrier-free)
产品规格：25 µg
产品说明：
Regulatory Status RUO
Other Names IL-6RA, CD126, IL6RA
描述
IL-6是一种细胞因子，在炎症、急性期反应、造血和免疫系统调节中发挥多种作用。IL-6以纳摩尔亲和力与IL-6Rα结合。复合物IL-6/IL-6Rα触发gp130的二聚化，其中受体-配体相互作用通过JAK/STAT途径诱导信号转导。gp130也是IL-11、CNTF、LIF、OSM、CT-1、CLC和IL-27的受体亚单位。gp130在不同类型的细胞中普遍表达。然而，gp130既不结合IL-6也不结合IL-6Rα，因此仅表达gp130的细胞对IL-6没有反应。α和gp130具有可溶性形式。可溶性IL-6Rα（sIL-6Rα）通过两种机制产生：从膜上脱落和差异剪接RNA的翻译。仅表达gp130的细胞可以对IL-6/sIL-6Rα复合物产生反应，这一过程被称为反式信号传导。炎症过程诱导中性粒细胞分泌IL-6R，复合物IL-6/sIL-6Rα激活内皮细胞产生MCP-1，从而吸引单核细胞。可溶性gp130（sgp130）可与IL-6和sIL-6Rα形成三元复合物，被认为是sIL-6Rα的天然抑制剂。在结肠炎、关节炎和特发性肺纤维化中检测到sIL-6Rα水平升高。sIL-6Rα的体内中和作用减轻了小鼠的肺纤维化。
IL-6 is a cytokine that plays multiple roles in inflammation, acute phase response, hematopoiesis, and regulation of the immune system. IL-6 binds to IL-6Rα with nanomolar affinity. The complex IL-6/IL-6Rα triggers dimerization of gp130, in which the receptor-ligand interaction induces the signal transduction though the JAK/STAT pathway. gp130 is also a receptor subunit for IL-11, CNTF, LIF, OSM, CT-1, CLC, and IL-27. gp130 is ubiquitously expressed in different types of cells. Nevertheless, gp130 does not bind IL-6 nor IL-6Rα, thus cells only expressing gp130 are unresponsive to IL-6. IL-6Rα and gp130 have soluble forms. Soluble IL-6Rα (sIL-6Rα) is generated by two mechanisms: by shedding from the membrane and by translation of a differentially spliced RNA. Cells expressing only gp130 can be responsive to the complex of IL-6/sIL-6Rα, and this process has been named trans-signaling. Inflammatory processes induce the shedding of IL-6R from neutrophils, and the complex IL-6/sIL-6Rα activates endothelial cells to produce MCP-1, which attracts monocytes. Soluble gp130 (sgp130) can be found in a ternary complex with IL-6 and sIL-6Rα, and it has been suggested to be the natural inhibitor of sIL-6Rα. Increased levels of sIL-6Rα have been detected in colitis, arthritis, and idiopathic pulmonary fibrosis. In vivo neutralization of sIL-6Rα attenuated pulmonary fibrosis in mice.
Product Details
Source
Human IL-6Rα, amino acids (Leu20-Asp358) (Accession# NM_000565), was expressed in insect cells.
Molecular Mass
The 339 amino acid recombinant protein has a predicted molecular mass of approximately 38.7 kD. The protein migrates at approximately 58 kD in DTT-reducing conditions and at 54 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 3.0 - 15 ng/ml, as determined by the ability of IL-6Rα to increase the growth arrest on mouse myeloid leukemia M1 cells induced by human IL-6 (20 ng/ml).
Application Bioassay
Antigen Details
Structure Monomer, soluble receptor.
Distribution
IL-6Rα is expressed in hepatocytes, neutrophils, macrophages, and lymphocytes. Soluble IL-6Rα is present in body fluids (urine and blood).
Function
Soluble IL-6Rα plays a key role in IL-6 trans-signaling, which is a process that allows the response to IL-6 on cells that do not express IL-6Rα. It increases the polarization of Th17 by IL-6. It is produced by proteolysis by ADAM17. Its shedding is induced by PMA, IL-1β, TNF-α, and apoptosis.
Interaction
Endothelial cells and a variety of cells expressing gp130.
Ligand/Receptor
IL-6 and sIL-6Rα/IL-6 complex bind cells expressing gp130.
Bioactivity
IL-6 promotes the growth arrest and differentiation of mouse myeloid leukemia M1 cells, and this effect is enhanced by sIL-6Rα.
Cell Type Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
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BioLegend 751504 Recombinant Human IL-6Rα (carrier-free)
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