BioLegend 715302 Recombinant H

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BioLegend 715302 Recombinant Human IL-7 (Animal-Free)
产品简介：
产品品牌：BioLegend
产品货号：715302
产品名称：BioLegend 715302 Recombinant Human IL-7 (Animal-Free)
产品规格：10 µg

产品说明：
Regulatory Status   RUO
Other Names
Interleukin 7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor

描述
IL-7最初被描述为一种基质衍生因子，能够在体外诱导前B细胞的生长。IL-7通过其受体（IL-7R）作用于多种细胞，IL-7R是一种由IL-7Rα（CD127）和其他细胞因子（IL-2、IL-4、IL-9、IL-15和IL-21）受体共享的共同γc链（CD132）组成的异二聚体。此外，IL-7Rα与TSLP共享。IL-7缺陷和IL-7Rα缺陷小鼠的产生和单克隆抗体阻断实验证实了IL-7对小鼠B细胞发育的需求。然而，严重联合免疫缺陷（SCID）患者IL-7受体α链的突变证实了IL-7对人类T细胞发育是必不可少的。然而，这些个体中B细胞的存在表明IL-7在小鼠和人类淋巴细胞发育中的作用存在重要差异。因此，尽管人类B细胞的发育似乎不需要IL-7，但未成熟的人类B细胞确实会对IL-7产生反应而增殖。然而，zui新的信息表明，在脐血和骨髓个体发育过程中，人类淋巴细胞生成中的IL-7依赖性增加。IL-7可与肝细胞生长因子（HGFβ）结合形成混合细胞因子（IL-7/HGFβ。混合细胞因子通过IL-7R（IL-7Rα加γc）和c-Met发出信号。IL-7具有胸腺抗凋亡作用，并诱导抗凋亡蛋白Bcl-2和Mcl-1的表达以及对促凋亡蛋白Bax和Bad的抑制。此外，IL-7是T细胞葡萄糖摄取的关键调节因子。TGF-β已被证明可下调人骨髓基质细胞的IL-7 mRNA和蛋白质分泌。此外，TGF-β抑制IL-7诱导的前B细胞增殖。
IL-7 was initially described as a matrix derived factor that can induce the growth of pre B cells in vitro. IL-7 acts on various cells through its receptor (IL-7R), which is a type of protein produced by IL-7R α (CD127) and other cytokines (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors share a common γ A heterodimer composed of c-chains (CD132). In addition, IL-7R α Share with TSLP. IL-7 defects and IL-7R α The production of defective mice and monoclonal antibody blocking experiments have confirmed the demand of IL-7 for mouse B cell development. However, in patients with severe combined immunodeficiency (SCID), the IL-7 receptor α The mutation of the chain confirms that IL-7 is essential for the development of human T cells. However, the presence of B cells in these individuals indicates significant differences in the role of IL-7 in mouse and human lymphocyte development. Therefore, although the development of human B cells does not seem to require IL-7, immature human B cells do respond to IL-7 and proliferate. However, the latest information indicates an increase in IL-7 dependence in human lymphocyte production during the ontogeny of cord blood and bone marrow. IL-7 can interact with hepatocyte growth factor (HGF) β） Combining to form mixed cytokines (IL-7/HGF) β。 Mixed cytokines pass through IL-7R (IL-7R α plus γ c) Signal with c-Met. IL-7 has an anti apoptotic effect on the thymus, inducing the expression of anti apoptotic proteins Bcl-2 and Mcl-1, as well as inhibiting pro apoptotic proteins Bax and Bad. In addition, IL-7 is a key regulatory factor for glucose uptake by T cells. TGF- β It has been proven to downregulate IL-7 mRNA and protein secretion in human bone marrow stromal cells. In addition, TGF- β Inhibition of IL-7 induced proliferation of pre B cells.

Product Details
Source
Human IL-7, amino acids Asp26-His177 (Accession# NM_000880.3), was expressed in E. coli.

Molecular Mass
The 153 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17.4 kD. The predicted N-terminal amino acid is Met.

Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation   Lyophilized, carrier-free.
Endotoxin Level    Less than 0.1 ng per µg of protein.

Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Activity
ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells.
Application   Bioassay

Application Notes
This product is reactive with human, mouse, and rabbit.

Antigen Details
Structure   Cytokine
Distribution
IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells.

Function
IL-7 induces proliferation of human immature B cells, and it is critical for T-cell development and peripheral T-cell homeostasis.

Interaction
IL-7Rα is expressed on immature B cells through the early pre-B stage. The IL-7Rα is widely expressed in T cells, CD4, CD8 naïve and memory cells, and dendritic cells.

Ligand/Receptor
IL-7Rα (CD127), common gamma chain (γc or CD132)
Cell Type    Hematopoietic stem and progenitors
Biology Area    Cell Biology, Stem Cells, Immunology
Molecular Family    Cytokines/Chemokines

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