BioLegend 686402 Purified anti

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BioLegend 686402 Purified anti-STAT1 Phospho (Ser727)
产品简介：
产品品牌：BioLegend
产品货号：686402
产品名称：BioLegend 686402 Purified anti-STAT1 Phospho (Ser727)
产品规格：100 µg

产品说明：
Clone   A15158B (See other available formats)
Regulatory Status   RUO
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype   Mouse IgG1, κ

描述
STAT1，也称为信号转导和转录激活因子1，是一种普遍表达的细胞质蛋白，并在细胞因子信号传导中被激活，包括IFN-α、IFN-γ、EGF、PDGF和IL-6。激活后，STAT1被受体相关激酶磷酸化，易位到细胞核，并作为转录因子发挥作用。STAT1的两种亚型，表观分子量分别为88和91kD，是替代RNA加工的结果。STAT1参与IFN介导的免疫反应，STAT1缺陷小鼠对细菌和病毒感染高度敏感。
STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Isotype Control   Purified Mouse IgG1, κ Isotype Ctrl
Verified Reactivity   Human, Mouse
Antibody Type   Monoclonal
Host Species   Mouse
Immunogen
Human STAT1 peptide phosphorylated at Ser 727.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography.
Concentration   0.5 mg/ml

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application    WB - Quality tested
ICFC, ICC, ChIP - Verified

Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.

Distribution
Translocates to the nucleus when phosphorylated.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.

Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area    Cell Biology, Signal Transduction
Molecular Family    Nuclear Markers, Phospho-Proteins

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